Contigs Built with Fingerprints, Markers, and FPC V4.7

Table 5.

Varying Error

Error Ctgs Bury (%) F− F+ Qs Order
None 145 + 9 69 156 − 9 36 4 (1) (66, 12, 0)
Ends 156 + 10 63 182 − 20 36 4 (1) (548, 93, 0)
Vector 133 + 9 67 153 − 17 38 24 (1) (212, 76, 0)
4% F+ 158 + 10 67 176 − 14 36 0 (0) (258, 59, 0)
4% F− 153 + 15 68 191 − 30 36 0 (0) (521, 78, 0)
Tol 174 + 17 51 257 − 73 30 0 (0) (249, 224, 0)
All 276 + 43 9 547 − 229 21 13 (5) (1520, 1792, 0)
  • (Ctgs) contigs; (F−) false-negative; (F+) false-positive; (Qs) questionable; (Tol) tolerance.

  • The data set has a 15× coverage, 10,015 clones, seven gaps, 145–185 kb lengths, one chimeric contig, and uses 7 tolerance and a 1e-10 cutoff.

  • Adds a band of 1387, 3695, and 3766 to 85%, 33%, and 33% of the clones, respectively.

  • The F+'s occurred at the ends of two contigs, so the incorrectly merged contig assembled without causing a stack, i.e., Q clones.

  • Adds or subtracts a tolerance of 0 through 7 for (16, 20, 18, 15, 12, 9, 6, 4) of the bands, respectively.

  • Uses all of the previous error.

This Article

  1. Genome Res. 10: 1772-1787

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