Contigs Built with Fingerprints, Markers, and FPC V4.7

Table 3.

Varying Coverage

Cov Gap Clones Bury(%) Largest (order) F+ clones
10× 18 6676 62 506 (4, 2, 0) 13 (3), 11 (2)
20× 3 13353 74 3529 (17, 2, 0) 33 (9), 18 (2)
30× 0 20030 79 5306 (15, 2, 0) 54 (9), 32 (2)
40× 0 26707 83 7110 (15, 2, 0) 72 (9), 44 (2)
50× 0 33384 85 9534 (37, 4, 0) 93 (9), 57 (2)
Cov 1e-08 1e-10 1e-10, CpM
Ctgs F− F+ Qs Ctgs F− F+ Qs Ctgs F− Qs
10× 135 144 29 30 (1) 193 216 28 2 (1) 92 80 4 (1)
20× 48 60 76 190 (3) 95 106 65 2 (1) 24 25 18 (1)
30× 22 28 262 1229 (4) 51 59 228 45 (1) 9 11 81 (1)
40× 15 19 524 674 (3) 34 38 465 62 (1) 5 7 365 (1)
50× 12 15 941 1086 (3) 27 28 778 204 (1) 4 3 724 (1)
  • (Cov) coverage; (CpM) cutoff plus marker; (Ctgs) contigs; (F−) false-negative; (F+) false-positive; (Qs) questionable.

  • The data set has perfect data, 145–185 kb clone lengths, a marker every 20 kb, and a tolerance of 7.

  • Percentage buried in which a buried clone has 90% or more shared bands with the parent clone.

  • The largest contig with no Q clones and its order triple.

  • The number of clones that have an F+ overlap for the 1e-08 and 1e-10 cutoff, respectively. In parentheses is the number of regions that have F+ overlaps. The number of F+ overlaps is the same for 1e-10 with or without markers.

  • The CpM rules are (1 1e-08) (2 1e-07) (3 1e-06).

  • There are four chimeric contigs, but the fourth does not have Q clones. The three F+'s occur at the end of two contigs and bridge the contigs with little difficulty, i.e., F+'s at the ends of contigs causing an incorrect merge may result in a number of extra bands and gaps that looks the same as error in the data.

This Article

  1. Genome Res. 10: 1772-1787

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