The RISA filtrator and densitometer. (A) Photograph of RISA filtrator and densitometer. (B) Schematic representation of RISA filtrator and densitometer. (C) Vacuum filtration sites and conveyer of RISA filtrator. In B, deep-well plates on DT are supplied at position DS1 on CDL. Eight DTs can be piled up on DS1. Filter plates for plasmid preparation are piled up in plate stacker (ST) and supplied to the plate separation apparatus (FD). Each filter plate is carried to the dispensing site (D). DT is conveyed to the culture transfer site (DS2), and all deep-well culture plates are scanned by RISA densitometer at the densitometory site (RD). After scanning, the next step is to obtain the appropriate volume of culture liquid from the deep-well plate at position A of DS2 on CDL by suction using the movable 96-channel autodispenser. The dispenser transfers samples to the filter plate at site 1 on FL. The filter plate is subjected to suction under vacuum at stage 1, then it conveyed to the next stage in a 30-sec interval. After transferring of the samples, the autodispenser is washed in an ethanol bath (EW) and water bath (WW). Twenty filter plates are piled up at plate-stacking apparatus (FS), and a set of 12 of the filter plates is conveyed to ST step by step. At the RD, site 1, and site 8, barcode readers detect the deep-well plate and filter plate IDs.
