A hybrid Kua–UEV gene in Homo sapiens genomic PAC clone dJ1185N5. (A) Prediction of the exon structure of humanKua (middle), supported by gene prediction algorithms (top), and alignments with expressed sequences (bottom). (B) Schematic representation of the relative arrangement of exons in the human Kua–UEV locus, based on predictions for clone dJ1185N5. (C) RT–PCR analysis showing expression of human Kua (K1F + KR), a hybridKua–UEV transcript (K2F + UR), and UEV1A (A + UR) in six cell lines. RT–PCR was performed with primers for the putative 5′ UTR (K1F), the 3′ UTR (KR), or the 5′ UTR of Kua (KR), and the end of exon C3 of UEV1 (UR), using as templates RNAs from the human cell lines HT-29 (lane1), SW480 (lane 2), HeLa (lane 3), SK—PC-3 (lane 4), T24 (lane 5), and HEL (lane 6). (Arrows) Specific amplification products; (asterisk) nonspecific amplification products. The faster-migrating band in the rightpanel corresponds to an alternative form of UEV1A (Sancho et al. 1998). (D) Expression analysis by RT–PCR of Kua–UEV hybrid transcripts and Kua-only transcripts on RNAs from cell lines Jurkat (lane 1), SK–NS-H (lane 2), N2A (lane3), PZ–HPV7, (lane 4), CA–HPV10 (lane 5), PC-3 (lane 6), and SK–PC-1 (lane 7). Amplification with primers for transcripts corresponding to the housekeeping S14r ribosomal protein was used to normalize for input RNA.
