A High-Resolution HAPPY Map of Dictyostelium discoideum Chromosome 6
Abstract
We have made a high-resolution HAPPY map of chromosome 6 ofDictyostelium discoideum consisting of 300 sequence-tagged sites with an average spacing of 14 kb along the ∼4-Mb chromosome. The majority of the marker sequences were derived from randomly chosen clones from four different chromosome 6–enriched plasmid libraries or from subclones of YACs previously mapped to chromosome 6. The map appears to span the entire chromosome, although marker density is greater in some regions than in others and is lowest within the telomeric region. Our map largely supports previous gene-based maps of this chromosome but reveals a number of errors in the physical map. In addition, we find that a high proportion of the plasmid sequences derived from gel-enriched chromosome 6 (and that form the basis of a chromosome-specific sequencing project) originates from other chromosomes.
[The sequence data described in this paper have been submitted to the GenBank data library under accession nos.G64996–G65046.]
Footnotes
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↵1 Corresponding author.
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E-MAIL bak{at}mrc-lmb.cam.ac.uk; FAX 44-1223-412178.
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Article and publication are at www.genome.org/cgi/doi/10.1101/gr.141700.
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- Received March 22, 2000.
- Accepted August 25, 2000.
- Cold Spring Harbor Laboratory Press
