Predicted genomic structure and expression analysis of DLK1. (A) Predicted genomic structure of DLK1. Black boxes and horizontal lines denote exons and introns, respectively. (*) shows the location of a single nucleotide polymorphism in exon 5 (position 148,740 of accession no. AL132711). (B) Parent-of-origin–dependent monoallelic expression of DLK1. The allelic expression of DLK1 was determined using a 108-d gestation conceptus heterozygous for the C/T polymorphism in exon 5. The maternal genotype is C/C, demonstrating paternal inheritance of the T allele in the conceptus. RNA analysis of the indicated tissues shows exclusive expression of the paternally derived T allele (i.e., C allele is unexpressed; arrowhead).
