Imprinting analysis of Msuit. F1 cDNA derived from fetal and adult tissues was sequenced from bidirectional crosses of Mus musculus musculus (129/Sv) and Mus musculus castaneus(CAST). A G/C (129/CAST) transcribed polymorphism identified in the genomic DNA at nucleotide 247 was used to distinguish the two alleles. (A) Expression analysis of Msuit in the brain, heart, intestine, kidney, testis, and ovary of F1 obtained from a cross of 129 (mother) and CAST (father). Genomic DNA sequences from each parent and from F1 are included. (B) Expression of Msuit in the brain, heart, spleen, testis, lung, liver, and kidney of F1 from the reciprocal cross. Genomic DNA sequences from paternal parent (129) and F1 are included.
