Physical and gene map of the VWS critical region. The VWS critical region is defined by genetic recombinants at D1S491 and D1S205 (open box at top). The CEPH YAC clone 785B2 contains both flanking markers in the original YAC contig (Schutte et al. 1996). STS content for this YAC and each of the BAC clones is indicated by long vertical lines. Restriction sites are indicated by short vertical bars and includeBssHII (B), MluI (M), NotI (N), andNruI (Nr). Parentheses indicate restriction sites that were absent on the indicated clone. The specific address for each BAC and PAC clone is shown. Sequencing of these clones is complete (thick line), partial (thick, shaded line), or only at their ends (thin lines). The sequence at the T7 (■) and Sp6 (●) ends for each BAC clone was determined. The presence and location of the 8-kb deletion/insertion polymorphism is indicated by an open rectangle. The order and orientation of genes, putative genes, and psuedogenes are indicated with arrowheads. For the putative genes VWS26,VWS37, VWS38, and VWS42, the direction of transcripton could not be deduced (flat bar). See text for definitions of genes, putative genes, and psuedogenes.
