Table 2.
Screening Libraries Using Various Single Locus Markers
| Markers | RPCI-21 | RPCI-22 | RPCI-23 | Locus |
| Comparative anchor-tagged sequences (CATS) | ||||
| Aminolevulinate, δ, syntase 1 (ALAS2) | 10 | 8 | 14 | X.63 |
| Biglycan (BGN)* | 7 | 9 | 10 | |
| Catalase (CAT) | 17 | 7 | 10 | 2.57 |
| Ceruloplasmin (ferroxidase) (CP) | 6 | 13 | 6 | 9.53 |
| Cytochrome P-450, subfamily XIX (CYP19) | 4 | 5 | 13 | 9.31 |
| Dopamine Receptor (DRD2) | 10 | 11 | 9 | 9.28 |
| Factor 9 (F9) | 8 | 6 | 11 | X.22 |
| Fibronectin 1 (FN1) | 9 | 9 | 23 | 1.36 |
| Glucosidase, β; acid (GBA)* | 11 | 8 | 24 | |
| Glucose-regulated protein (78 kD) (GRP78) | 13 | 12 | 3 | 2.23 |
| Insulin-like growth factor binding prot. 1 (IGFBP1) | 16 | 14 | 17 | 11.02 |
| Immunoglobulin heavy chain gene cluster (IGH)* | 12 | 17 | 16 | |
| Mineralcorticoid (aldosterone) receptor (MLR) | 7 | 10 | 20 | 8.35 |
| Moloney murine sarcoma oncogene homol. (MOS) | 8 | 10 | 16 | 4.00 |
| Myeloperoxidase (MPO) | 7 | 7 | 19 | 11.50 |
| Neurofibromin 1 (NF1) | 13 | 16 | 18 | 11.46 |
| Paired box homeotic gene (PAX8) | 17 | 4 | 14 | 2.10 |
| Avian reticuloendotheliosis viral onc. hom. (REL) | 9 | 11 | 21 | 11.13 |
| Thy-1 cell surface Antigen (THY1) | 4 | 15 | 19 | 9.26 |
| Exon | ||||
| HPRT | 10 | 12 | 10 | X.17 |
| End probes from BAC/PAC | ||||
| 3-17D3-T | 7 | 10 | 23 | 2.57 |
| 3-209M19-T | 9 | 12 | 6 | 2.57 |
| 1-439F16-T | 11 | 14 | 15 | 11.02 |
| 3-20C9-T | 7 | 20 | 11 | 11.02 |
| 3-233F12-S | 6 | 13 | 6 | 8.35 |
| 3-458o24-S | 7 | 12 | 15 | 8.35 |
| EN21 | 5 | 9 | 3 | |
| EN31 | 11 | 4 | 17 | |
| Average number of positive clones | 9.3 | 10.6 | 13.9 |
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The locus column indicates the mouse chromosome location of each probe. For example, the HPRT gene is located on chromosome X, at the 17-cM position. The BGN, GBA, and IGHprobes are marked with an asterisk (*). No homology was found to theBGN sequence using the database, whereas the GBAand IGH PCR products showed homologies against glucocerebrosidase (GCB) and MHC class IIH2-IE-α genes. However, the original names are used here.
