Bacterial Artificial Chromosome Libraries for Mouse Sequencing and Functional Analysis

Table 1.

Mouse Libraries Described in this Paper

RPCI-21
DNA source: 129S6/SvEvTac female spleen
Cloning vector: pPAC4
Segment Cloning enzyme Total clones Number of plates Noninsert clones (%) Insert size (kb) Genomic representation
1 MboI 125,318 336 0.5 127 5.3
2 MboI 128,899 336 0.5 147 6.3
 Total 254,217 0.5 137 11.6
RPCI-22
DNA sources: 129S6/SvEvTac female spleen
Cloning vector: pBACe3.6
1 EcoRI 102,914 288 5.5 155 5.3
2 EcoRI 109,039 288 0.1 154 5.6
 Total 211,953 2.7 154 10.9
RPCI-23
DNA source: C57BL/6J kidney and brain
Cloning vector: pBACe3.6
1 EcoRI 80,661 240 11.3 198 5.3
2 EcoRI 89,973 240 1.3 197 5.9
 Total 170,634 6.0 197 11.2

This Article

  1. Genome Res. 10: 116-128

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