Pooled Genotyping of Microsatellite Markers in Parent–Offspring Trios

Table 2.

Results from the Spiking Experiment

Marker (original pool vs. %) Pvalues
ΔAIP Peak heights not corrected Peak heights corrected Individual genotyping
D4S431
vs. 5% 0.83 0.99 0.97 0.89
vs. 10% 0.21 0.31 0.079 0.22
vs. 15% <0.001 0.001 <0.001 <0.001
D4S230
vs. 5% 0.93 1 0.85 0.97
vs. 10% 0.80 0.97 0.31 0.39
vs. 15% 0.13 0.58 0.001 0.009
DRD5
vs. 5% 1 1 0.65 0.95
vs. 10% 0.98 0.96 0.15 0.32
vs. 15% 0.97 0.88 0.036 0.026
D4S1602
vs. 5% 0.56 0.57 0.51 0.91
vs. 10% 0.066 0.25 0.095 0.4
vs. 15% 0.01 0.012 <0.001 0.039
D4S2375
vs. 5% 1 0.9 0.9
vs. 10% 0.92 0.57 0.7
vs. 15% 0.47 0.14 0.24
  • Presented are the values for statistical significance based on ΔAIP, from peak heights as produced by CLUMP (before and after correction), and from the true genotyping results. For each marker, three values correspond to 5%, 10%, and 15% enrichment of one individual's DNA. In bold are the significant results.D4S2375 did not require correction.

This Article

  1. Genome Res. 10: 105-115

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