Pooled Genotyping of Microsatellite Markers in Parent–Offspring Trios

Table 1.

Results from Pools and Individual Genotyping of 110 Trios

Marker ΔAIP Pvalues
Peak heights Individual genotyping
Not corrected Corrected CLUMP ETDT
MSX1 1 0.87 0.45 0.30 0.34
D4S2375 0.9 0.85 0.68 0.75
D4S2374 0.3 0.34 0.37 0.78 0.68
D4S431 1 1 0.75 0.26 0.15
DRD5 1 0.9 0.76 0.46 0.30
D4S1602 0.51 0.26 0.12 0.50 0.33
D4S3007 0.6 0.9 0.9 0.4 0.27
D4S230 1 0.68 0.15 0.85 0.38
  • Simulated significance levels of the ΔAIP.

  • Peak heights method before and after correction for stutter and differential amplification.

  • The allele frequencies of probands and controls (the untransmitted alleles) from the true genotyping were analyzed with CLUMP to present a statistical result that is more closely related to the one produced by the peak height method (which uses CLUMP).

  • Extended transmission/disequilibrium test (ETDT) from individual genotyping of the families.

This Article

  1. Genome Res. 10: 105-115

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