Pooled Genotyping of Microsatellite Markers in Parent–Offspring Trios

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Figure 5.
Figure 5.

Relationship between stutter band heights and allele sizes for markerD4S230. The sizes (vertical axis) refer to the ratio between the height of the stutter band and the height of the primary peak. The three lines correspond to stutter bands that are 1, 2, and 3 bp units shorter than the correct fragment size. Each point is estimated as the mean of 4–10 observations. Alleles 4, 5, and 7 were rare, and their stutter bands could not be plotted. The stutter bands of allele 6 appear not to fit the trend of the other alleles, but it was also rare (∼3%) and was less reliable to construct.

This Article

  1. Genome Res. 10: 105-115

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