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Method: Nondestructive enzymatic deamination enables single-molecule long-read amplicon sequencing for the determination of 5-methylcytosine and 5-hydroxymethylcytosine at single-base resolution

Zhiyi Sun,
Romualdas Vaisvila,
Laura-Madison Hussong,
Bo Yan,
Chloé Baum,
Lana Saleh,
Mala Samaranayake,
Shengxi Guan,
Nan Dai,
Ivan R. Corrêa, Jr.,
Sriharsa Pradhan,
Theodore B. Davis,
Thomas C. Evans, Jr.,
and Laurence M. Ettwiller

Genome Res. February 2021 31: 291-300; Published in Advance January 19, 2021, doi:10.1101/gr.265306.120

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