Searching journal content for articles similar to Mikheenko et al..

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  1. Perspective: Challenges in identifying mRNA transcript starts and ends from long-read sequencing data
    • Ezequiel Calvo-Roitberg,
    • Rachel F. Daniels,
    • and Athma A. Pai
    Genome Res. November 2024 34: 1719-1734; doi:10.1101/gr.279559.124
    .... Overview of current long-read RNA sequencing technologiesTwo main companies, Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT) offer commercial solutions for long-read RNA sequencing.PacBio. In this technology, a single-stranded circularized cDNA molecule is generated by ligating hairpin...
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  2. Method: Contrasting and combining transcriptome complexity captured by short and long RNA sequencing reads
    • Seong Woo Han,
    • San Jewell,
    • Andrei Thomas-Tikhonenko,
    • and Yoseph Barash
    Genome Res. October 2024 34: 1624-1635; Published in Advance September 25, 2024, doi:10.1101/gr.278659.123
    .... 2023; Lucas and Novoa 2023; Marx 2023). The most commonly used platforms, Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT), offer RNA sequencing with read lengths typically varying between a few hundred to a few thousand bases long, depending on the technology and the protocol used...
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  3. Review: Understanding isoform expression by pairing long-read sequencing with single-cell and spatial transcriptomics
    • Natan Belchikov,
    • Justine Hsu,
    • Xiang Jennie Li,
    • Julien Jarroux,
    • Wen Hu,
    • Anoushka Joglekar,
    • and Hagen U. Tilgner
    Genome Res. November 2024 34: 1735-1746; doi:10.1101/gr.279640.124
    ...as a template (Eid et al. 2009). This was followed by the release of the Oxford Nanopore Technologies (ONT) platform, which measures changes in current induced by the movement of a nucleic acid molecule through a nanopore. Since then, both technologies’ capabilities have found numerous applications spanning...
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