Experimental validation of the sequence model in predicting sgRNA efficiency. (A) A SURVEYOR gel picture (top) and a bar chart (bottom) showing the indel rates of the sgRNAs predicted to be inefficient (low sequence score) or efficient (high sequence score). The sgRNAs were selected to target the AAVS1 locus. The experiment was conducted in 293T cells. (B) A scatter plot showing the correlation of the predicted sequence scores and the protein knockout efficiency for sgRNAs targeting AR and FOXA1 in LNCaP-abl cells. The knockout efficiency is measured as the percentage of reduction in protein level upon sgRNA infection.