A schematic view of procedures for sgRNA selection and categorization. (A,B) Venn diagrams showing the overlap of essential genes between human HL-60 and KBM-7 cells (A) and two biological replicates in mouse ESC JM8 cells (B). (C–E) Scatter plots showing the log₂ fold-change of sgRNA abundance in negative selection upon cell growth. (C) sgRNAs targeting essential ribosomal genes in Wang data. (D) sgRNAs targeting essential nonribosomal genes in Wang data. (E) sgRNAs targeting essential genes in Koike-Yusa data. The dashed lines represent the threshold chosen to determine efficient and inefficient sgRNAs.