Verification of the expression data retrieved from the microarray comparison of flowers versus leaves with real time reverse transcriptase PCR.
Quantitative RT-PCR | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Microarray | Raw (Ct) | Normalized ΔCt | |||||||||
| EST ID | Annotation | Average ratio F/L | Flower | Leaf | Flower | Leaf | Ratio F/L | ||||
| G0000100033H3 | Polyphenol oxidase | 0.41 | 18.44 | 25.99 | 3.61 | 11.51 | 0.004 | ||||
| G0000100002G1 | Flavonoid 1-2 rhamnosyltransferase | 0.73 | 24.38 | 26.34 | 9.55 | 11.86 | 0.202 | ||||
| G0000300001A5 | Non-specific lipid transfer protein | 1.49 | 22.08 | 19.61 | 7.25 | 5.13 | 4.347 | ||||
| G0000300017G4 | Cytochrome P450 | 0.32 | 23.07 | 25.66 | 8.24 | 11.18 | 0.130 | ||||
| G0000700010E10 | T-complex protein | 0.75 | 21.12 | 20.99 | 6.29 | 6.51 | 0.859 | ||||
[i] The raw threshold cycle (Ct) values were normalized against a ubiquitin standard to get normalized ΔCt values. The expression differences in the leaf and flower sample were calculated from the formula 2ΔCt flower / ΔCt leaf. F = flower, L = leaf.