Table 4.

Estimates of the Level of Constraint in Introns of 38 Loci From D. melanogaster and D. simulans, Computed Using the Three-Lineage Method





Substitutions

Lineage
Data set
bp Per locus
Observed (SEM)
Expected (SEM)
Constraint (SEM)
melanogaster Complete 204 7.05 (1.07) 7.17 (1.15) 0.010 (0.11)
simulans 6.49 (1.04) 7.82 (1.59) 0.15 (0.14)
melanogaster Splice sequences omitted 161 5.87 (0.99) 5.45 (0.96) –0.087 (0.13)
simulans 5.39 (0.95) 5.90 (1.36) 0.065 (0.15)
melanogaster 5′ bases 3–6 7.8 0.184 (0.074) 0.326 (0.045) 0.44 (0.21)
simulans 0.052 (0.036) 0.370 (0.057) 0.86 (0.10)
melanogaster 3′ bases 3–16 27.3 1.003 (0.200) 1.112 (0.196) 0.084 (0.19)
simulans


1.034 (0.200)
1.205 (0.201)
0.12 (0.21)