Maximum Correlation of Expression and Solubility to Molecular Properties
LCCa | |||
|---|---|---|---|
| Expression | Solubility | Parameter | |
| −0.31 | −0.17 | Signal Peptideb | |
| −0.24 | −0.20 | GRAVY (hydrophobicity)c | |
| −0.24 | −0.11 | Transmembrane Helicesd | |
| −0.14 | −0.09 | Number of cysteines | |
| −0.17 | −0.08 | Anchor peptide | |
| −0.12 | −0.06 | Prokaryotic membrane lipoprotein lipid attachment sitee | |
| 0.16 | 0.12 | PDB identity | |
a Linear correlation coefficients (LCC). A cut-off of 0.10 is used for the absolute value of LCC for expression in reporting analysis results in the table.
b Signal Peptides (http://www.cbs.dtu.dk/services/SignalP/) (Nielsen et al. 1997).
c GRAVY (Grand Average of Hydropathicity) value for a peptide or protein is calculated as the sum of hydropathy values of all of the amino acids, divided by the number of residues in the sequence using the EXPASY site (http://ca.expasy.org/tools/protparam.html) (Kyte and Doolittle 1982; Gasteiger et al. 2003).
d Transmembrane Helices (http://www.cbs.dtu.dk/services/TMHMM-2.0/) (Krogh et al. 2001).
e Prokaryotic membrane lipoprotein lipid attachment site predicted by PROSITE (Bairoch et al. 1997).