Table 2.

Target Loci of Dnmts Detected in RLGS Confirmed by Vi-RLGS and the Methylation-Sensitive Real-Time PCR Method

Spot no.a
Locus
Gene symbolb
Gene name
CpG island
12 15B2 repeat (LTR element) — yes
15 5G2 D130017N08Rik RIKEN cDNA D130017N08 gene yes
17 11E2 Cbx4 chromobox homolog 4 no
18 8B1.1 — — no
21 12D1 LOC238316 predicted gene yes
25 10B4 4732470K04 predicted gene yes
30 13A5 LOC238623 predicted gene yes
31 7B3 Dbx1 developing brain homeobox 1 yes
32 9A5.1 D130038B21 Rik RIKEN cDNA D130038B21 gene yes
34 18C AA987150 predicted gene yes
43 2H3 LOC329560 predicted gene yes
47 19C3 Trim8 tripartite motif protein 8 yes
53 5B1 BC037112 gene with protein product no
73 9A4 D730048106Rik RIKEN cDNA D730048106 gene yes
76 2H3 — — no
77 11A4 AL732390.10.1.195612.21298.172306 predicted gene no
79 10C1 Agpat3 1-acylglycerol-3-phosphate O-acyltransferase 3 yes
87 14D1 Baiap1 BAI1-associated protein 1 no
88 10B4 Mm.338763 (unigene) — no
93 7D1 Bnc basonuclin yes
95 14C1 MGC38922 predicted gene yes
97 7A3 Rps19 ribosomal protein S19 yes
99 17E3 Cox7a21 cytochrome c oxidase subunit VIIa polypeptide 2-like yes
109 13A5 — — yes
111 7F3 hmm37743 predicted gene no
115 7A2 LOC381956 predicted gene yes
144 13B1 Tgfbi transforming growth factor, beta induced no
152 19C3 Pik3ap1 phosphoinositide-3-kinase adaptor protein 1 yes
161 5B2 Man2b2 mannosidase 2, alpha B2 yes
166 5F Cit citron no
167 4D3 LOC230868 predicted gene no
173 11A4 Odz2 odd Oz/ten-m homolog 2 no
178 2H1 Bfzb-pending basic FGF repressed, zinc binding protein no
212 14D2 repeat (DNA element) — yes
225 11B1.3 A830006N08 predicted gene yes
231 16C2 — — yes
234 11B5 — — no
235 8E2 — — no
236
10C1
Pcnt2
pericentrin 2
no

a Spot numbers indicate the position in RLGS profile shown in Fig. 1A.

b Symbols are shown if the Notl site of the fragment is located within 10 kb of the gene.