The Relative Solvent Accessibility of ABPαβγ ω+ Sites That Map to the Recombinant Cat Allergen Fel DI (PDB 1PUO)
1PUO chain 1 | ABPα | 1PUO chain 2 | ABPβγ | |||||
|---|---|---|---|---|---|---|---|---|
| All sites | Mouse and rat ω+ | Mouse only ω+ | All sites | Mouse and rat ω+ | Mouse only ω+ | |||
| No. of residues | 68 | 4 | 5 | 67 | 29 | 17 | ||
| No. buried | 5 | 0 | 0 | 6 | 3 | 1 | ||
| No. intermediate | 22 | 0 | 0 | 19 | 6 | 2 | ||
| No. exposed | 41 | 4 | 5 | 42 | 20 | 14 | ||
| Buried (%) | 7.4 | 0.0 | 0.0 | 9.0 | 10.3 | 5.9 | ||
| Intermediate (%) | 32.4 | 0.0 | 0.0 | 28.4 | 20.7 | 11.8 | ||
| Exposed (%) | 60.3 | 100.0 | 100.0 | 62.7 | 69.0 | 82.4 | ||
[i] The solvent accessibility values of cat Fel dl residues equivalent to ABPαβγ ω+ residues were calculated from the structure coordinates using the DSSP algorithm (Kabsch and Sander 1983). Relative accessibility scores were partitioned into three states: buried (<9% relative accessibility), intermediate (9%-35% relative accessibility), and exposed (≥36% relative accessibility), as described previously (Rost and Sander 1994).