Description of In Silico Assignment of Microsatellite Markers to the Rat Genome
RNO | Markersa | e-PCR mappinga | Average spacing (kb)b | Inconsistencies (%)c |
|---|---|---|---|---|
| 1 | 1149 | 834 | 293.7 | 26 (3.1%) |
| 2 | 948 | 632 | 371.0 | 6 (0.9%) |
| 3 | 675 | 497 | 303.7 | 10 (2.0%) |
| 4 | 692 | 470 | 352.9 | 2 (0.4%) |
| 5 | 694 | 475 | 323.6 | 3 (0.6%) |
| 6 | 540 | 403 | 348.2 | 14 (3.5%) |
| 7 | 573 | 414 | 316.6 | 7 (1.7%) |
| 8 | 580 | 425 | 277.0 | 12 (2.8%) |
| 9 | 453 | 310 | 335.3 | 5 (1.6%) |
| 10 | 686 | 480 | 215.4 | 5 (1.0%) |
| 11 | 284 | 196 | 397.3 | 3 (1.5%) |
| 12 | 307 | 205 | 215.0 | 4 (2.0%) |
| 13 | 476 | 316 | 311.9 | 4 (1.3%) |
| 14 | 355 | 258 | 377.8 | 3 (1.2%) |
| 15 | 381 | 266 | 375.9 | 4 (1.5%) |
| 16 | 315 | 223 | 372.8 | 2 (0.9%) |
| 17 | 416 | 311 | 281.2 | 2 (0.6%) |
| 18 | 325 | 234 | 342.5 | 2 (0.9%) |
| 19 | 253 | 172 | 297.6 | 0 (0.0%) |
| 20 | 194 | 123 | 389.4 | 1 (0.8%) |
| Unassigned | 1289 | 853 | ||
| Total | 11,585 | 8097 | 270.4 | 115 (1.4%) |
a The number of markers used for e-PCR mapping to the rat genome sequence includes all available microsatellites, including 1289 markers previously defined by a D-Number nomenclature (e.g., D0Got, D0Mco) that could not be assigned to a rat chromosome by genetic or RH mapping
b The average distance between markers in the genomic sequence was calculated with the chromosome length determined in the June 2003 rat genome assembly, and includes above-described markers that are only mapped by e-PCR
c Details of markers that are assigned to different chromosomes by linkage or RH mapping and e-PCR are available in our data repository (http://www.well.ox.ac.uk/rat_mapping_resources)