Comparison of Expression by Different Vector and Bacterial Strain Combinationsa
pET15G with BL21-AI | pET15G with BL21(DE3)pLysS | pET21G with BL21-AI | pET21G with BL21(DE3)pLysS | |
|---|---|---|---|---|
| No. of proteins expressed | 70 | 25 | 65 | 11 |
| % ORFs in plate | 79.5% | 28.4% | 73.9% | 12.5% |
a From expression data for the 88 ORFs in plate 18.