Table 1.

Methylation-specific PCR primer sequence and amplicon size

Primer set
Forward primer, 5′ → 3′
Reverse primer, 5′ → 3′
Size (bp)
ACTB TGGTGATGGAGGAGGTTTAGTAAGT AACCAATAAAACCTACTCCTCCCTTAA 130
CFTR-M GTTTTGGGTTTGGCGGATTTTGACGC CCCGCAAATAAACGACAATCGCGAC 142
CFTR-U GGTTTTGGGTTTGGTGGATTTTGATGT CATCCCACAAATAAACAACAATCACAAC 146
DBC1-M ACGCGATCCCTTTAAATACTCGTACG GAGGAGAGACGGGAGGTCGTTTCG 131
DBC1-U ATATACACAATCCCTTTAAATACTCATACA GGAGGAGAGATGGGAGGTTGTTTTG 136
DLK1-M TCCGAAAAAATCTACGACCCAAATTCG AGACGGGTATAGGTATTTCGCGAGC 132
DLK1-U TCTCCAAAAAAATCTACAACCCAAATTCA GTTAGATGGGTATAGGTATTTTGTGAGT 137
EYA4-M TACGGAGATTACGGCGGCGTTATTC AACGCGAAAAACGACGACGCGCGA 150
EYA4-U GGGTATGGAGATTATGGTGGTGTTATTT CCAAAAACACAAAAAACAACAACACACAA 158
NPY-M TTAAAACCCTCTAACCGAAAACTTCCG ACGATTAGCGCGGTATTTTCGTCGG 131
NPY-U
CTTTAAAACCCTCTAACCAAAAACTTCCA
TTTTTATGATTAGTGTGGTATTTTTGTTGG
138