CIITA activates core promoter mutants
Co-transfection with: | ||||
|---|---|---|---|---|
| Promoter construct | Control vector (%) | CIITA expression vector (%) | Fold activation | |
| −416WT | 17.5 ± 1.4 | 97.5 ± 7.5 | 5.6 | |
| −416ATG−6 | 2.2 ± 0.5 | 32.5 ± 3.5 | 14.8 | |
| −416ATG−24 | 1.3 ± 0.4 | 20.2 ± 3.4 | 15.5 | |
| −416ATG−42 | 19.9 ± 2.3 | 92.4 ± 2.3 | 4.3 | |
[i] The ability of CIITA to activate wild type (WT) or mutant (out-of-frame ATG) −416CAT reporter constructs was examined in transiently transfected HeLa cells, which were co-transfected with either a control or CIITA expression vector. Data are expressed as CAT activity, corrected for the pSV2LUC internal transfection control; standard errors derive from triplicate samples.