Features of the mini-Tn5-luxCDABE mutant library in P. aeruginosa PAO1
Feature | N |
|---|---|
| Random mini-Tn5-luxCDABE mutants isolated | 18,500 |
| Mutants arrayed and stored | 9984 |
| Samples processed through inverse PCR | 4608 |
| DNA sequencing reactions | 3325 |
| Total mapped insertion sites | 2519 |
| Insertions within a gene | 2275 |
| Intergenic insertions | 244 |
| Transcriptional luxCDABE fusions | 1253 |
| Nonredundant insertion sites | 1932 |
| Unique ORFs inactivated | 1284 |
| Unique intergenic insertions | 174 |
| Unique transcriptional luxCDABE fusions | 784 |
| Predicted PAO1 ORFs | 5570 |