BACs and BIBACs Fingerprinted and Used for the Soybean Physical Map
Libraries | Cloning site | No. of clones fingerprinted | Average insert size (kb) | No. of clones used in mapping | Average no. of bands/clone | Redundancy genome equivalent |
|---|---|---|---|---|---|---|
| Forrest | ||||||
| BIBAC-H | HindIII | 30,720 | 125 | 27,221 | 27.63 | 3.052× |
| BIBAC-B | BamHI | 21,504 | 125 | 20,181 | 23.43 | 2.262× |
| BAC-E | EcoRI | 30,720 | 157 | 28,620 | 39.12 | 4.030× |
| Faribault | ||||||
| BAC | EcoRI | 1142a | 120 | 1125 | 28.46 | 0.121× |
| Williams 82 | ||||||
| BAC | HindIII | 860a | 150 | 854 | 32.03 | 0.115× |
| Combined libraries | 84,946 | 78,001 | 9.580× |
a The BACs were identified with 267 SSR markers and 105 RFLP markers by the laboratories of R. Shoemaker, Iowa State University and N. Young, University of Minnesota (Marek et al. 2001) from the soybean cv. Williams 82 (Marek and Shoemaker 1997) and cv. Faribault (Danesh et al. 1998) BAC libraries. The distribution of the markers in the soybean genetic map (Cregan et al. 1999a) were from Marek et al. (2001)