Table 1.

HT Cloning Efficiency Is Related to the Size of the Target Fragment

IGR size
Attempted
Cloned
Success rate
0-500 1548 1454 94
500-1000 1901 1644 86
1000-1500 1214 909 75
1500-2000 791 474 60
>2000 3510 2057 59
Total
8964
6538
73

[i] The success rate of the cloning procedure was estimated based on the detection of the correct size insert in at least one of the two colonies examined.