Table 1.

Summary of Recombinational Cloning Strategies in Terms of Demonstrated Efficiency, Adaptability, and Compatibility (See Text), Relative to Currently Available Full-Length cDNA Resources

N- & C- term
Efficiency
Adaptability
Compatibility
FL-cDNA No No No No
Gap repair Yes Yes No No
UPS Yes Yes Yes No
MAGIC Yes Yes Yes No
Gateway
Yes
Yes
Yes
Yes

[i] References to alternative strategies are as follows: gap repair (Orr-Weaver et al. 1983; Ma et al. 1987; Oldenburg et al. 1997), UPS (Liu et al. 1998), MAGIC (Paddison et al. 2004).