Plasma Lipid Concentrations in the Female Mice From Various B6 × NZB Crosses and Their Parental Strains Before and After High-Fat Diet
Total cholesterol | HDL | Non-HDL | |||||||
|---|---|---|---|---|---|---|---|---|---|
|
Before
|
After
|
Before
|
After
|
Before
|
After
| ||||
| C57BL/6J (n = 5) | 73±4 | 234±10 | 61±4 | 64±7 | 13±3 | 169±13 | |||
| NZB/B1NJ (n = 5) | 145±12a | 333±30b | 114±8a | 182±17a | 31±17 | 151±25 | |||
| F1 (n = 5) | 97±6 | 273±11 | 76±6 | 100±2 | 21±3 | 173±10 | |||
| N2 (n = 104) | 83±1 | 247±4 | 63±1 | 56±1 | 21±1 | 190±4 | |||
|
F11 (n = 349)
|
102±2
|
370±7
|
81±1
|
84±1
|
24±0
|
276±8
| |||
[i] Plasma from female B6, NZB, (B6 × NZB) F1 mice, and (B6 × NZB) × B6 N2 mice (12 weeks old and fed high-fat diet for 4 weeks) was measured for total cholesterol concentrations with a commercial colorimetric enzymatic assay, and HDL concentrations were measured after apolipoprotein B—containing lipoproteins were selectively precipitated with polyethylene glycol. Non-HDL represents the difference of total cholesterol and HDL. Plasma from (B6 × NZB) F11 mice (10 weeks old and on high-fat diet for 6 weeks) was measured for LDL, VLDL, and HDL concentrations with an enzymatic assay after gel filtration using a Superose 6 column. Non-HDL represents the total of LDL and VLDL concentrations. Values represent mean ± SEM (mg/dL) aP<0.01, bP<0.05, compared with B6 and F1