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Table 7.

Signal analysis of an example microarray

		Number of spots		635 nm		532 nm
				Feature		Background		Feature		Background
				Avg.	SD	Avg.	SD	Avg.	SD	Avg.	SD
ApiscDNAs	7329	3.42	0.47	2.23	0.05	3.43	0.48	2.15	0.04
Exogenous control 1	16	2.40	0.07	2.24	0.05	2.35	0.07	2.15	0.04
Exogenous control 2	16	2.43	0.07	2.23	0.04	2.41	0.06	2.15	0.04
Exogenous control 3	16	2.32	0.08	2.23	0.05	2.26	0.08	2.15	0.04
Exogenous control 4	16	2.38	0.06	2.24	0.04	2.32	0.05	2.15	0.04
Exogenous controls 5–48	43	2.49	0.20	2.23	0.05	2.47	0.22	2.15	0.05
All controls spots	107	2.43	0.15	2.23	0.05	2.39	0.17	2.15	0.04

	635 nm	532 nm
# Apis cDNAs > background + 1 SD	7308	7311
# Apis cDNAs > background + 2 SD	7300	7305
# Apis cDNAs > control spots + 1 SD	7029 (96%)	7035 (96%)
#Apis cDNAs > control spots + 2 SD	6647 (91%)	6631 (90%)

[i] All inteneity values were log10 transformed. Feature and background readings are indicated for each cDNA spot (based on median pixel intensity) for 635 and 532 nm wavelengths. Average (Avg.) and standard deviation (SD) are indicated. Exogenous controls are described in Methods.