Table 6.

Different Random Sets

Set Ctgs F− F+ Qs Order Order/no Qs[iii]
142 + 1102 − 5959112 (13)(2770, 3400, 9)(186, 220, 0)
243 + 295 − 5010848 (12)(2861, 3414, 1)(395, 475, 0)
338 + 188 − 4991145 (14)(3024, 3634, 25)(414, 458, 1)
444 + 492 − 44123243 (16)(2871, 3416, 10)(391, 458, 1)
4[iv] 27 + 035 − 92368 (1)(111, 16, 0)(84, 11, 0)
4[v] 6 + 07 − 231222 (1)(508, 422, 1)(4, 1, 0)

[i] (Ctgs) contigs; (F−) false-negative; (F+) false-positive; (Qs) questionable.

[ii] Each of the first four sets is a different random selection of 16,692 clones from the same pool, a 25× coverage with clone lengths of 130–190 kb, and one marker per 45 kb, and there is one chimeric contig. The error includes end fragments, 4% F+, 4% F, and the tolerance distribution of (16, 20, 18, 15, 12, 9, 6, 4). All the data sets were assembled with a 7 tolerance, 1e-10 cutoff with cutoff plus marker table (1 1e-08, 2, 1e-07, 3 1e-06). On the average, 17% of the clones are buried. The data sets assembled without markers resulted in 143, 155, 137, and 138 contigs, respectively.

[iii] The second order triplet is for the contigs with no Q clones.

[iv] The data set is the same as the previous one but without error. The set with error has 28 F+ clones with an average of 7 F+ overlaps per clone, whereas the set without error has 32 F+ clones with an average of 13 F+ overlaps per clone.

[v] The data set is the same as the previous one that has no error but is assembled with a tolerance of 0 and a cutoff of 1e-12. The largest contig with no F+'s has 4738 clones and two out-of-order pairs.