Validation of Microarray Data by PCR and Sequence Analysis
| Strain | Cj0008 | Cj1585 | Cj0055–Cj0058 | Cj0565–Cj0569 | ||||||||
| array | PCR | Sequencing | Array | PCR | Sequencing | Array | PCR | Sequencing | Array | PCR | Sequencing | |
| 81116 | − | − | − | − | −[ii] | −[iii] | − | − | − | − | −[ii] | −[iv] |
| 81-176 | + | + | + | − | −[ii] | −[iii] | + | + | + | − | −[ii] | −[iv] |
| X | − | − | − | + | + | + | + | + | + | + | + | + |
| G1 | − | − | − | + | + | + | + | + | + | + | + | + |
[i] A + or − indicates the presence or absence, respectively, or a gene indicated by microarray data, alongside the subsequent analysis data.
[ii] Putative insertion identified by PCR, rather than a deletion.
[iii] Sequence analysis suggests the presence of an anaerobic dimethyl sulphoxide reductase ortholog adjacent to Cj1584.
[iv] Analysis of sequence data adjacent to Cj0564shows no matches with any database entry.