Table 4.

Phylogenetically Restricted Sequences of the Apicomplexa

Reference (dbEST ID no.) Contig ID[i] (no. of ESTs) P value for apicomplexan genera P value of closest nonapicomplexan Putative identification dbEST/ GenBank accession nos.
Apical antigens/adhesins
467174TQ-753 (92) Neospora(10−22)100 dense granule antigen U72991
467174 Plasmodium (10−3) Pf-EMP1 U27338
467347 Sarcocystis(10−15)10−1 microneme protein L08892
467347 Eimeria(10−14) A22655
466328TQ-702 (2) Plasmodium(10−12)10−1 apical membrane antigen I L27503
475702TQ-1456 (2) Plasmodium(10−14)10−10 stippled structure-assoc. antigen U10121
Developmental antigens
466318TQ-11 (8) Eimeria(10−50)10−1 19-kD sporozoite antigen M59500
620297 Plasmodium(10−13)10−7 gametocyte-specific antigen Pfs97 Z37724
489378 Plasmodium(10−11)10−1 trophozoite antigen (Ser kinase?) Z11832
487888 Plasmodium(10−11)10−6 M83793
574122TQ-254 (12) Eimeria(10−31)10−10 developmental mRNA M98840
574122 Plasmodium(10−20) M29000
Metabolism, signaling, structural
513090 Plasmodium(10−20)10−8 RNA polymerase III (large subunit) M73770
653609TQ-1975 (4) Plasmodium(10−13)10−9 HMG protein L31630
653609 Babesia(10−13) M81360
574303 Plasmodium(10−11)10−4 P-type ATPase U39298
574303 Cryptosporidium(10−10) U65981
487624 Eimeria(10−23)10−14 transhydrogenase L08392
467004 Eimeria(10−15)10−6
489375 Eimeria(10−11)10−2
488081TQ-1442 (11) Neospora(10−28)10−4 14-3-3 regulatory protein U31542
465739 Neospora(10−23)10−16
571541 Neospora(10−67)10−33
Unknown function
467430TQ-627 (9) Cryptosporidium(10−17)10−9 EST (identity unknown) AA224687
465760TQ-377 (4) Plasmodium(10−39)10−10 aspartate-rich protein U46930

[i] Available from the Toxoqual database listing the consensus sequence for aligned EST contigs http:/daphne.humgen.upenn.edu/toxodb/