[i] ^aWatson and Crick refer to the direct and reverse strands of the DNA. These columns indicate the number (No.) and the percentage of genes in each orientation.

[ii] ^bOrthologs of essential genes in S. cerevisiae.

[iii] ^cFour introns not predicted in EMBL files were detected in Sakl0C and Sakl0H chromosomes by comparative analysis with the L. thermotolerans genome. The new gene models are: SAKL0C03542g join(334775..334797, 334858..335686); SAKL0C05742g complement(join(546510..547835, 547914..547937)); SAKL0C10780g join(978865..978870,979002..980159); SAKL0H15114g complement(join(1308968..1311883, 1311996..1312019)).