Accuracy of Genotype Discrimination and Comparison of Radioactive and Fluorescent Detection in Minisequencing on Microarrays for Four Polymorphic Y-Chromosomal SNPs
| Tat[i] (T/C) R = T/(T+C) | M9 (G/C) R = G/(G+C) | SRY10831[i] (G/A) R = G/(G+A) | M17 (T/G) R = T/(T+G) | |||||
| Sample genotype[ii] | T | C | G | C | G | A | T | G |
| Fluorescent detection | ||||||||
| Variation[iii] | 0.90–0.93 | 0–0.07 | 0.91–1.0 | 0.06–0.40 | 0.75–1.0 | 0–0.04 | 0.70–0.80 | 0–0.08 |
| Average | 0.93 | 0.04 | 0.97 | 0.16 | 0.87 | 0.02 | 0.74 | 0.04 |
| Power of genotype discrimination[iv] | 23 | 6.1 | 44 | 19 | ||||
| Radioactive detection | ||||||||
| Variation | 0.99–1.00 | 0.00–0.05 | 0.93–0.99 | 0–0.09 | 0.94–1.0 | 0–0.04 | 0.92–0.99 | 0–0.05 |
| Average | 1.00 | 0.02 | 0.96 | 0.03 | 0.96 | 0.02 | 0.97 | 0.02 |
| Power of genotype discrimination[iv] | 50 | 32 | 48 | 49 | ||||
[i] Detection from complementary strand.
[ii] Data from ten samples of each genotype was used, except for the fluorometric measurements of M9 C allele, SRY10831 G allele and M17 T allele where only five samples were used.
[iii] The range of variation between the lowest and highest R values.
[iv] Raverage of alleles1/Raverage of alleles2.