Sequences of Primer Sets Used in this Study
| DBP5′ (5′-flanking region) | 5′-TTCCAAGCTCCAGAGTCATGGACC-3′ |
| 237 bp | 5′-TGGCAGAGGCGTGAGATGGTTTAAG-3′ |
| DBP E1[i](exon 1) | 5′-TACCACTTTTACATGGTCAC-3′ |
| 157 bp | 5′-GAATTCCTGTAAATGGTCAC-3′ |
| DBP (exon 4) | 5′-ACCTCAGCACTGTCTGCCAAGT-3′ |
| 212 bp | 5′-TGATTAGCATATTCCTTTGGATCTTTCCTG-3′ |
| DBP (intron 6) | 5′-AGCCCTCCAAACTGTTTCAACCCC-3′ |
| 172 bp | 5′-ACACGGAACTGTGAAGTTGCCCAG-3′ |
| E9[i] (exon 9) | 5′-ATCTCCTTTTCTCCCTCATGC-3′ |
| 239 bp | 5′-AGGCAGTGAGCAAGTTTTGG-3′ |
| E11[i] (exon 11) | 5′-ACATGTAGTAAGACCTTAC-3′ |
| 249 bp | 5′-GATTGGAGTGCATACGTTC-3′ |
| EP1[ii] | 5′-TGGGTCAAGTCTGCTTACCATCTG-3′ |
| 203 bp | 5′-GTTGGGAGTGGCTGTTGATTTG-3′ |
| EP2[ii] | 5′-TTGGGAAAGAAAGGTTGCCTAAC-3′ |
| 252 bp | 5′-ATTCAGTTTGCATCCATTGATTTT-3′ |
| AFP (exon 12) | 5′-GCTGACATTATTATCGGACACTTATGT-3′ |
| 224 bp | 5′-TCTTGCTTCATCGTTTGCAGCGC-3′ |
| AFM (1790–1878)[iii] | 5′-CAACCCTGCTGTGGACCAC-3′ |
| 88 bp | 5′-GCACATATGTTTTATCAGCTTT-3′ |
[i] Yuasa et al. (1995).
[ii] EP1 and EP2 are the end sequences of the inserts of PAC clones 230N6 and 45P24, respectively.
[iii] The number in parenthesis is the nucleotide number of the AFM cDNA (Lichenstein et al. 1994). The sizes of the amplified fragments are indicated below the names of primers.