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Table 3.

FP–TDI Assay with Synthetic Templates Using Different Dye Terminators

Templates	BFL–ddA (mP)[ii]	TMR–ddC (mP)[ii]	ROX–ddG (mP)[ii]	BTR–ddU (mP)[ii]
CF508–48A	38	43	77	174
	37	53	73	175
	31	36	78	174
	35	49	82	170
CF508–48C	20	50	214	32
	19	37	209	27
	20	56	215	25
	14	38	207	26
CF508–48G	23	247	84	23
	24	266	80	30
	22	253	75	23
	15	262	74	21
CF508–48T	113	52	81	32
	106	41	68	39
	108	59	81	30
	103	32	76	28
Avg. ctrl.	25	46	86	28
s.d.Ctrl.	8.2	8.8	4.6	5.0
Avg. net chg.[iii]	83	211	134	145

[i] Positive reactions are in boldface type.

[ii] FP measurements for BFL were made with excitation at 480 nm and emission at 520 nm; for TMR the excitation was 535 nm and emission was 590 nm; for BTR the excitation was 591 nm and emission was 635 nm; for ROX the excitation was 580 nm and emission was 620 nm.

[iii] Net change over average of control.