Table 3.

EST–SNPs Confirmed by Fluorescence-Based Sequencing

Contig[iii] EST–SNP sequence context[iv] Frequency[v]
1AAGCTGTGGC(C/T)ACACCTTCCC4/2
1CTTCCCGCTC(G/C)TCTGCTGCTG4/2
2CCATAAATGC(G/A)CTAAGATAAA5/1
2AATTAAAGAA(C/T)AATAATGTTC5/1
3TTTTAACAAA(G/C)TAATCTTCAC5/1
4ATAAGATTAT(T/A)ATAATATATA5/1
5TTCAGTTTTG(A/T)GGCTCATGGG5/1
6CAGTCTGTAT(C/T)TTCCAAAAAG5/1
7GAGTCGAAAT(G/C)TGATTCTTCA4/2
7GAATGCAGTC(C/T)GTCATATGAC4/2
7TGACCACTAA(C/T)TTGCATGTGA4/2

[i] Summary of SNPs confirmed by fluorescent-based sequencing.

[ii] The contig sequences and their corresponding GenBank accession numbers can be found in the NCBI SNP database under the submitter handle debnick SNPS-981209-A and SNPS-981209-B.

[iii] More than one SNP per contig was confirmed in contigs 1, 2, and 7.

[iv] Context surrounding the substitution noted between parentheses (major allele/minor allele).

[v] Total of six chromosomes sequenced (three individuals).