Table 3.
Detection of Polymorphisms in the Known Genes by PCR Amplification
| Gene | Acc. no | Primer origin[i] | Primer pairs[ii] | Fragment size (bp) |
Intron
(bp) | Polymorphism | LG | Homology[iii] | Location in human[iv] | Location in zebrafish (LG)[v] |
Intron
(bp)
Polymorphism
LG
Homology
Location in human
Location in zebrafish (LG)
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|
[i] Accession numbers are shown for the medaka nucleotide sequences used for designing primer pairs.
[ii] Primer pairs depicted in italics were first primer sets used for sequencing but not for segregation analysis.
[iii] Homology search was conducted against the nucleotide sequences of the genes from the HNI strain.
[iv] The locations of the human gene loci in the human genome were taken from the homepage (http://gdbwww.gdb.org/gdb/) or were referred to in Lyons et al. (1997).
[v] The locations of the zebrafish gene loci in the zebrafish genome were referred to Postlethwait et al. (1998).