Table 3.

Characteristics of Different Sources of Mapped Chromosome 7 STSs

STS source[i] Mapped to YAC contig[ii] YAC-based connectivity to Genethon genetic markers[iii] Negative YAC screen[iv]
same YAC same contig no connection
Random568 (26%)500 58 10 6
YAC insert ends790 (37%)690 80 20 0
Genetic markers438 (20%)157 17  4  4
Genes/ESTs354 (17%)293 43 18 13
 Total2150 1640 (87%)198 (10%)52 (3%)23

[i] The chromosome 7-specific STSs can be broadly classified into four major categories: those derived from sequences corresponding to random segments of chromosome 7 DNA [e.g., isolated by flow-sorting, subcloning, or Alu–PCR amplification of human DNA from chromosome 7-containing monochromosomal hybrid cell lines (Green et al. 1991a; Green 1993; Bouffard et al. 1997)], YAC insert ends, genetic (microsatellite) markers, and genes and ESTs.

[ii] Number (and relative percentage) of STSs mapped to the YAC contigs listed in Table 1, indicated for each STS source.

[iii] YAC-based physical connectivity between the mapped chromosome 7 STSs and Genethon genetic markers is indicated. A total of 1890 chromosome 7 STSs (2150 mapped STSs minus the 260 mapped Genethon genetic markers) were analyzed for their YAC-based connection to 1 or more of the 260 Genethon genetic markers localized on the contigs listed in Table 1. Indicated are the numbers of STSs from each source connected to a Genethon genetic marker(s) by the same YAC(s) or same YAC contig (but not the same YAC), as well as those with no YAC-based connection to a Genethon genetic marker (Green et al. 1994). The total (and relative percentage) for each of the three indicated categories is also provided.

[iv] Number of STSs for which no positive YAC could be identified, indicated for each STS source.