Characteristics of Different Sources of Mapped Chromosome 7 STSs
| STS source[i] | Mapped to YAC contig[ii] | YAC-based connectivity to Genethon genetic markers[iii] | Negative YAC screen[iv] | ||||
| same YAC | same contig | no connection | |||||
| Random | 568 (26%) | 500 | 58 | 10 | 6 | ||
| YAC insert ends | 790 (37%) | 690 | 80 | 20 | 0 | ||
| Genetic markers | 438 (20%) | 157 | 17 | 4 | 4 | ||
| Genes/ESTs | 354 (17%) | 293 | 43 | 18 | 13 | ||
| Total | 2150 | 1640 (87%) | 198 (10%) | 52 (3%) | 23 | ||
[i] The chromosome 7-specific STSs can be broadly classified into four major categories: those derived from sequences corresponding to random segments of chromosome 7 DNA [e.g., isolated by flow-sorting, subcloning, or Alu–PCR amplification of human DNA from chromosome 7-containing monochromosomal hybrid cell lines (Green et al. 1991a; Green 1993; Bouffard et al. 1997)], YAC insert ends, genetic (microsatellite) markers, and genes and ESTs.
[ii] Number (and relative percentage) of STSs mapped to the YAC contigs listed in Table 1, indicated for each STS source.
[iii] YAC-based physical connectivity between the mapped chromosome 7 STSs and Genethon genetic markers is indicated. A total of 1890 chromosome 7 STSs (2150 mapped STSs minus the 260 mapped Genethon genetic markers) were analyzed for their YAC-based connection to 1 or more of the 260 Genethon genetic markers localized on the contigs listed in Table 1. Indicated are the numbers of STSs from each source connected to a Genethon genetic marker(s) by the same YAC(s) or same YAC contig (but not the same YAC), as well as those with no YAC-based connection to a Genethon genetic marker (Green et al. 1994). The total (and relative percentage) for each of the three indicated categories is also provided.
[iv] Number of STSs for which no positive YAC could be identified, indicated for each STS source.