The contigs generated with Velvet and Minimus were scaffolded using paired-end genomic reads (see Methods)

[i] Base accuracy was determined by examining variation in the invariable splice site junctions surrounding coding exons. Structural accuracy was estimated identifying scaffolds that unequivocally align to two locations (see Results). These accuracy measures represent an upper-level estimate on potential errors in our assembly, since it is expected that these two strains might differ slightly.