Markup | Genome Research

Table 3.

Epistatic Interactions Detected by the EpistatProgram and Confirmed by ANOVA

Marker 1		Marker 2		`Epistat`		ANOVA
Marker 1		Marker 2		interaction LLR	interaction P-value	marker 1 P-value	marker 2 P-value	R²	d.f.	interaction F-value	interaction P-value
3% Ethanol intake
D2Mit61	D17Mit51[iii]	8.2	0.0067	0.17	0.004[iii]	0.068	1, 25	7.9	0.0094
D3Mit86[ii]	D13Mit147	12.8	0.0002	0.02[ii]	0.65	0.13	1, 31	13.2	0.00099
D6Mit55	D13Mit35	9.3	0.0042	0.30	0.95	0.029	1, 35	9.7	0.0036
D7Mit7[ii]	D13Mit44	9.7	0.0031	0.009[ii]	0.71	0.13	1, 43	12.2	0.0011
D10Mit194	D14Mit52	21.6	0.0005	0.76	0.21	0.0034	1, 27	11.0	0.0026
D13Mit97	DXMit69	11.4	0.0005	0.90	0.40	0.00027	1, 46	11.9	0.0012
D14Mit82	D18Mit33	12.4	0.0009	0.53	0.66	0.014	1, 27	17.0	0.00032
3% Ethanol preference
D1Mit19	D16Mit6	8.1	0.0101	0.51	0.48	0.018	1, 22	9.8	0.0049
D1Mit48	D12Mit194	9.1	0.0026	0.39	0.89	0.022	1, 31	9.0	0.0052
D1Mit37	D15Mit96	9.4	0.0011	0.08	0.17	0.10	1, 27	14.1	0.00083
D3Mit25	D6Mit36	10.6	0.0008	0.69	0.41	0.0041	1, 35	10.4	0.0028
D3Mit10	D17Mit6	8.7	0.0045	0.37	0.91	0.027	1, 29	9.0	0.0054
D4Mit4	D8Mit271[ii]	11.2	0.0005	0.39	0.01[ii]	0.021	1, 33	8.4	0.0067
D4Mit58[ii]	D19Mit35	8.7	0.0020	0.03[ii]	0.39	0.11	1, 33	10.8	0.0024
D6Mit36	D13Mit35	8.6	0.0021	0.46	0.42	0.017	1, 30	15.1	0.00053
D8Mit271	D9Mit218	20.3	0.0003	0.22	0.63	0.043	1, 32	13.1	0.00099
D9Mit306	D19Mit10	12.1	0.0032	0.43	0.96	0.017	1, 35	10.5	0.0026
D11Mit199	D16Mit6	10.9	0.0044	0.50	0.53	0.014	1, 31	10.7	0.0026
10% Ethanol intake
D2Mit61	D18Mit144	8.6	0.0007	0.18	0.25	0.052	1, 32	15.9	0.00037
D6Mit36	D13Mit35[iii]	29.2	<0.0001	0.42	0.03[iii]	0.020	1, 30	15.3	0.00049
D7Mit15	D13Mit44	9.1	0.0012	0.94	0.79	0.00019	1, 27	15.6	0.00051
D8Mit56	D16Mit71[ii]	9.1	0.0045	0.41	0.02[ii]	0.018	1, 35	7.8	0.0080
D11Mit184	D19Mit10	8.5	0.0025	0.25	0.92	0.032	1, 38	8.9	0.0050
10% Ethanol preference
D1Mit48	D12Mit20	16.8	0.0010	0.23	0.38	0.041	1, 34	12.9	0.0010
D1Mit37[ii]	D15Mit96	17.3	0.0005	0.03[ii]	0.79	0.15	1, 27	19.1	0.00016
D2Mit61	D18Mit144	9.1	0.0013	0.22	0.06	0.044	1, 32	16.4	0.00031
D3Mit89[iii]	DXMit69	9.2	0.0030	0.007[iii]	0.23	0.14	1, 43	12.8	0.00089
D5Mit214	D8Mit190	41.7	0.0005	0.89	0.33	0.00053	1, 36	8.1	0.0071
D6Mit36	D13Mit35[iii]	18.7	0.0082	0.50	0.01[iii]	0.014	1, 30	21.4	0.000068
D6Mit201	D11Mit199	16.3	0.0057	0.97	0.45	0.00005	1, 35	7.7	0.0088
D10Mit162	D13Mit34	16.8	0.0002	0.89	0.17	0.00050	1, 42	9.0	0.0045
D12Mit46	D15Mit193	10.0	0.0063	0.73	0.78	0.0032	1, 36	7.8	0.0085

[i] LLR, log likelihood ratio. R² represents portion of phenotypical variation controlled by the interacting loci.

[ii] Single marker showing significant effect on a phenotype in theEpistat analysis (P < 0.05), but with no linkage to the trait in the Mapmaker analysis (LOD < 0.8).

[iii] Single marker showing significant effect on a phenotype in theEpistat analysis (P < 0.05) and located in a region of significant or suggestive linkage. LOD scores are: 2.1 (D17Mit51 and 3% ethanol intake), 1.0 (D13Mit35and 10% ethanol intake), 1.4 (D13Mit35 and 10% ethanol preference), and 1.7 (D3Mit89 and 10% ethanol preference).