Distribution of ESTs and SAGE Tags in Five Alternatively Polyadenylated Genes[i]
| Gene | Site no. | No. of ESTs | SAGE tag | No. of SAGE tags | Distance from site 1 |
| PDXK | 1 | 35 | AGTGTCCGGC | 186 | |
| Pyridoxal kinase | 2 | 9 | TAAACAGTTG | 16 | 2.8 kb |
| 3 | 23 | TGGATGTTTG | 12 | 6.2 kb | |
| CSTB | 1 | 130 | TTGGTGCTTT | 27 | |
| Cystatin B | 2 | 4 | CAAAACAGAA | 22[ii] | 1.4 kb |
| AGPAT3 | 1 | 15 | GCGGAGCTGG | 12 | |
| 1-acylglycerol-3-phosphate | 2 | 14 | GGGTCTCCTG | 99 | 0.75 kb |
| O-acyltransferase 3 | 3 | 15 | AACACTCGCC | 52 | 1.9 kb |
| 4 | 13 | ACAGCCACTG | 62 | 2.5 kb | |
| TMEM1 | 1 | 5 | TAATTGTAGC | 12 | |
| Transmembrane protein 1 | 2 | 5 | GGATGTCCTA | 14 | 1.5 kb |
| 3 | 7 | GTTTACTATG | 12 | 1.8 kb | |
| SMT3H1 | 1 | 11 | CCGACCACAA | 121 | |
| SMT3(suppressor of mlf two 3, | 2 | 11 | TAACCTCGGG | 57 | 0.85 kb |
| yeast) homolog | 3 | 85 | TTCTTCTCGT | 263 | 1.1 kb |
[i] For five representative genes from Table 2, the number of EST contributing to the definition of the most prominent polyadenylation sites was counted, NIaIII SAGE tags immediately upstream of the polyadenylation sites were deduced, and the cumulative representation of the tags in SAGE libraries was determined for each of the major polyadenylation sites. The sites are listed in the 5′ to 3′ direction relative to the transcript, including the distance from the 5′ most site for those lying downstream.
[ii] This SAGE tag is not unique to the CSTB gene; it also occurs in a transcript from gene AF5Q31 (ALL1 fused gene from 5q31).
[iii] SAGE, serial analyses of gene expression.