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Table 3.

Correlation Between Microarray Expression Analysis and Expression Studies in EOC Using Conventional Techniques Such as Northern Blot, RT-PCR or Immunohistochemistry

Accession no.	Gene symbol	Name	Data reported in literature	Expression values and reliability scores^a
Accession no.	Gene symbol	Name	Data reported in literature	NOV-31	TOV-81D	TOV-112D	OV-90	TOV-21G
Z15115	TOP2B	topoisomerase 2 β	Northern blot analysis revealed variable expression in 15 solid ovarian tumors (Withoff et al. 1999). Intertumoral variability of TOP2B mRNA levels was detected by RNAse protection assay and no significant increase in expression was detected in tumors compared to normal ovaries (Cornarotti et al. 1996).	49 P	47 P	117 P	53 P	116 P
M85079	TGFBR2	transforming growth factor beta receptor type 2	Expression of TGFBR2 was detected in 14 cell lines by RT–PCR (Hu et al. 2000). Northern blot analysis revealed differential expression of TGFBR2 in four EOC cell lines and one solid tumor (Manderson et al, 2000). Expression was detected in 92% of tumors by RT–PCR (Bartlett et al. 1997).	−53 A	−70 A	−67 A	−170 A	−62 A
R41937	MLH1	MutL (E. coli) homolog 1	Immunohistochemistry revealed positive staining in a large fraction of malignant cells in 54 solid tumors (Samimi et al. 2000).Colella et al (1998) were unable to detect MLH1 protein by Western blot in four of 20 tumors and 5 of 9 cell lines.	10 A	20 M	34 P	22 P	20 P
X87838	CTNNB1	catenin β 1	Four of twenty-seven tumors showed positive staining by immunohistochemistry, all four harbored mutations in the gene (Sagae et al. 1999). Expression of CTNNB1 was found to be variable among 34 adenocarcinomas of the ovary by immunohistochemistry (Davies et al. 1998).	192 P	98 P	153 P	155 P	335 P
R36644	ACVR2B	activin A receptor, type IIB	Expression was observed by RT–PCR in 100 percent of tumor samples (Zheng et al. 1998). Western blot detected ACVR2B protein in OVCAR-3 and SKOV3 (Ito et al. 2000).	44 M	29 A	100 P	92 A	53 M
M81933	CDC25A	cell division cycle 25A	CDC25A mRNA was detected by RT–PCR in nine of 14 cell lines (Hu et al. 2000).	43 P	16 A	90 P	60 A	36 A
H59260				4 A	−6 A	12 A	5 A	−4 A
R43361	RYK	RYK receptor-like tyrosine kinase	In situ hybridization revealed minimal to absent expression of RYK in the	29 P	39 P	33 A	92 P	64 P
X69970			surface epithelium of normal ovaries. Expression was elevated primarily in the epithelium of malignant tumors (Wang et al. 1996).	25 P	39 M	31 P	69 P	27 M
Z29090	PIK3CA	phosphatidylinositol 3-kinase catalytic subunit, alpha isoform	Increased transcription detected by quantitative PCR in one ovarian cancer cell line (Shayesteh et al. 1999).	10 A	−3 A	11 A	3 A	14 A
R23889	TFRC	transferrin receptor	Immunohistochemistry revealed heterogenous expression of TFRC	254 P	392 P	114 P	780 P	283 P
X01060			(Berchuck et al. 1990).	80 P	237 P	45 P	288 P	151 P
H60074				10 A	15 A	24 A	6 A	−29 A
M11507				7 A	−8 A	7 A	−1 A	−4 A
H26419	APOD	apolipoprotein D	18 of 68 ovarian tumors stained positively for APOD expression (Vazquez et al. 2000).	28 A	−6 A	19 A	29 A	33 A

[i] Numeric expression values before standardization and the reliability score for each expression value were generated by the Affymetrix GeneChip software based on hybridization signals for probe sets representing each EST.