Markup | Genome Research

Table 3.

Loci that Map to Nonhomologous Chromosomes on Sheep, Cattle, and Goat Linkage Maps

Locus	Sheep	Predicted cattle/goat	Cattle[ii]	Goat	Comment
\LSCV12	2	8		16	Primers amplify the same size microsatellite in sheep and goats; primers contain some microsatellite sequence; goat position from Web site as noted in Schibler et al. (1998) map paper.
\BM9248 [iii]	2, 20	8, 23	2, 12, 13^M		Primers amplify multiple microsatellite band sets of varying sizes in sheep and cattle.
\BMS1915 [i]	3, 14	11, 18	16^M		Primers amplify two microsatellite band sets in sheep of similar size to cattle bands; microsatellite band sets overlap.
\HEL12 [iii]	4	4	20^M,I		Lots of background; microsatellite band set is faint; sheep bands slightly larger than cattle bands.
EAA[iv]	6	6	15^M,R		Protein polymorphism.
\BM4439	8	9	15^M		Cattle has only 19 informative meioses; sheep bands much larger than cattle bands.
\BM8115 [i] [iii]	13	13	21^M		A low annealing temp (48°C/50°C) was used to amplify sheep DNA. Amplified a constant product as well as a polymorphic band set. Sequencing of both products revealed that the sequence of the constant product was similar to the bovine BM8115 sequence but that the (TG)_n repeat had been replaced by a (T)_n repeat. The other sequence was unrelated to bovine BM8115.
\ILSTS010 [iii]	5, 14	7, 18	12^M		No stutter pattern associated with sheep bands; probably not a microsatellite.
\BMS2840	16	20	7^M		Similar sizes for sheep and cattle bands.
\GMBT11	17	17	26^I		VNTR
EAM[iv]	18	21	23^M,I		Sheep protein typing; cattle typed by PCR; cattle has null allele and only 21 informative meioses.
EAC[iv]	20	23	18^M,R		Protein polymorphism; only 18 informative meioses in sheep but shows no linkage to any chromosome 14 marker.
\OARHH22	21	29	29^M	16	Reverse primer for goat differs from sheep primer.
\MCM373	22	26	2^M		Large size difference between sheep and cattle microsatellite bands; may not be the same marker.
\INRA104 [iii]	15, 22	15, 26	5^I		Primers amplify multiple microsatellite band sets in sheep.
\HEL6	23	24	1^M,I		Products of similar size in sheep and cattle.
\MAF48	X	X	5^M	19	Sheep bands slightly larger than cattle bands.

[i] Alternate primer sets used in sheep derived from cattle sequences in GenBank.

[ii] M, MARC97; I, IBRP97; R, IRRF95.

[iii] The sequence of the primers to amplify sheep DNA has been confirmed by sequencing PCR products.

[iv] Sheep and cattle erythrocyte antigens may not be homologous.