Top 15 Genes Whose Expression Profiles Correlated with Differential Expression of TPO Among 11 AML Samples
| Gene description | Probe | Z-score | P-value[ii] | t-test[iii] | W. r.s.[iv] | Relation to TPO and/or hematopoiesis |
| Thrombopoietin (TPO) | L36051 | −9.39 | <0.0001 | 0.0971 | 1.000 | TPO supports megakaryopoiesis, most important regulator of platelet production (Caen et al. 1999). |
| Jagged 1 | U73936 | 6.26 | <0.0001 | 1.0000 | 1.0000 | Jagged 1 signaling through notch 1 plays a role in hematopoiesis (Schroeder and Just 2000). |
| Carboxypeptidase (MAX.1) | J04970 | −5.81 | <0.0001 | 1.0000 | 1.0000 | MAX.1 associated with monocyte to macrophage differentiation and is expressed in AML cells (Rehli et al. 1995). |
| Dynamin 1 | L07807 | −5.27 | 0.0010 | 1.0000 | 1.0000 | Dynamin 1 induced by Grb2 when monocytes stimulated with M-CSF (Kharbanda et al. 1995). |
| Neutrophil gelatinase B-associated lipocalin (NGAL) | X99133 | 5.24 | 0.0011 | 1.0000 | 1.0000 | NGAL mainly expressed in myeloid cells (Bundgaard et al. 1994), NGAL specific granules are marker for neutrophil maturation (Le Cabec et al. 1997). |
| Beta 1 integrin D | U33880 | −5.12 | 0.0021 | 1.0000 | 1.0000 | TPO up-regulates adhesion of hematopoetic progenitors to fibronectin through activation of integrin alpha4beta1 and alpha5beta1 (Gotoh et al. 1997). |
| Sp4 transcription factor | X68561 | −5.06 | 0.0030 | 1.0000 | 1.0000 | Sp4 not well characterized but closely related to Sp1 (Suske 1999), TPO activates several Sp1-dependent genes during megakaryopoiesis (Wang et al. 1999). |
| Prothrombin | M17262 | 4.86 | 0.0082 | 1.0000 | 1.0000 | Thrombin cleaves TPO to various isoforms (Kato et al. 1997), thrombin and TPO may co-regulate myeloid differentiation (van Willigen et al. 2000). |
| Wilms tumor 1 | X69950 | 4.65 | 0.0236 | 1.0000 | 1.0000 | WT1 inhibits differentiation of myeloid progenitor cells (Tsuboi et al. 1999). |
| LIM-homeobox domain (hLH-2) | U11701 | −4.61 | 0.0284 | 1.0000 | 1.0000 | hLH-2 has a role in control of cell fate decision and/or hematopoietic proliferation (Pinto do et al. 1998). |
| Mitochondrial creatine kinase | J04469 | −4.61 | 0.0284 | 1.0000 | 1.0000 | ? |
| Thrombospondin 2 (TSP2) | HG896– HT896 | 4.61 | 0.0286 | 1.0000 | 1.0000 | TSP2 inhibits tumor growth and angiogenesis (Streit et al. 1999), close relative thrombospondin 1 is negative regulator of megakaryopoiesis (Chen et al. 1997; Touhami et al. 1997). |
| Lysyl hydroxylase 2 (PLOD2) | U84573 | 4.49 | 0.0507 | 1.0000 | 1.0000 | ? |
| Serotonin receptor | M83181 | 4.33 | 0.1039 | 1.0000 | 1.0000 | Serotonin secretion stimulated by TPO (FontenayRoupie et al. 1998). |
| Karyopherin beta 3 | U72761 | 4.30 | 0.1192 | 1.0000 | 1.0000 | ? |
[i] Dataset from Golub et al. (1999).
[ii] P-value computed from Z-score using a modified Bonferroni's correction.
[iii] P-value obtained from t-test with unequal variances using a modified Bonferroni's correction. When equal variances were assumed, the P-value for TPO was 0.1105 and theP-values for the other genes did not change.
[iv] P-value obtained from Wilcoxon rank sum using a modified Bonferroni's correction.