Table 3.

BLAST vs. FISH for BACs with Interchromosomal Duplications: Multiple Thresholds for a Postive BLASTSignal

Statistic Minimum sum of bases for positive chromosome[ii]
2500 5000 10000 20000 40000
Minimum percent identity for BLASTHSPs90% BLAST chr number24020114810453
% BLAST chr over FISH chr[i] 107% 90% 66% 46%24%
% FISH chr identified byBLAST  58% 53% 44% 34%17%
% BLAST chr confirmed by FISH0.470.510.590.650.68
94%Sum ofBLASTNUM 216183140 9252
%BLAST chr over FISH chr[i]  96% 82% 63% 41%23%
% FISH chr identified byBLAST  54% 50% 43% 30%17%
% BLAST chr confirmed by FISH 49% 53% 60% 65%69%
96% BLASTchr number166152118 8147
%BLAST chr over FISH chr[i]  74% 68% 53% 36%21%
% FISH chr identified byBLAST  46% 43% 38% 26%16%
% BLAST chr confirmed by FISH 54% 55% 63% 64%68%
 98% BLASTchr number119105 78 5336
%BLAST chr over FISH chr[i]  53% 47% 35% 24%16%
% FISH chr identified byBLAST  36% 33% 25% 19%13%
% BLAST chr confirmed by FISH 62% 65% 69% 75%75%
 99% BLASTchr number 70 57 46 3932
%BLAST chr over FISH chr[i]  31% 25% 21% 17%14%
% FISH chr identified byBLAST  22% 19% 16% 14%12%
% BLAST chr confirmed by FISH 63% 68% 72% 77%81%

[i] FISH identified 224 chromosome signals.

[ii] HSPs were summed within 400-kb sequence segments.