Table 3.

pKa Values of Ionizable Groups in Proteins Used for the Estimation of pI

Terminal carboxyl3.1
Aspartic and glutamic acid4.4
Histidine6.5
Terminal amino8.0
Cysteine8.5
Tyrosine or lysine10.0
Arginine12.0

[i] The values were taken from Stryer (1995).